Article

A Pilot Study on the Relationship of the Cytoskeleton Components with Normal Cellular Functions and Injuries in Malignant and Neuro-Degenerative Multi-Factor Disorders, Using Biological Systems with Rat, Mouse and Human Origin

Author : Iskra Sainova, Ilina Valkova, Elena Nikolova, Bistra Alexieva, Angel Baldziev, Angel Alishev and Marin Nenchev

The main goal of the current study was directed to evaluation of technologies for precise identification of changes in the cellular components and compartments, composed of bio-molecules and intra-molecular structures was directed to investigation of the importance of these proteins and peptides, as well as of the intra- and extra-cellular interactions between them, for in the normal functions of the tissues and organs, but also of respective abnormalities for the development of pathological events. The main goal of the current study was directed to evaluation of technologies for precise identification of changes in the cellular components and compartments, composed of bio-molecules and intra-molecular structures, based on differences in their electron density. Complex biological systems from human, rat and mouse, were used. In the highly differentiated mature cells in the composition of different tissues and organs, these abnormalities have been proved as main reason for abnormal functions, leading to development of diseases and disorders on organism level. These injuries in the cytoskeleton components have been suggested as reason for abnormal mitosis in the actively-dividing cells from the same individuals (as different types of undifferentiated leucocytes and their progenitors), subsequently leading to chromosomal aberrations. As one of the key molecules in the intra- and extra-cellular inter-molecular interactions by cascade regulatory pathways, in which these elements participate, has been proved the tri-peptide Glutathione, in particular, its reduced form GSH. For a better understanding of the physiological function of cytoskeleton components, several different methods for their quantification and identification were developed and tested, including methods for 3D-imagination of cells and sub-cellular components (chromosomes), with and without additional laser irradiation, were developed and tested. Future investigations on direct and/or indirect influence of these components on the function of other biological molecules by cascade regulatory mechanisms with their participation by intra- and extra-cellular interactions are necessary.


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