Antibacterial Activity of Lawsonia inermis (Sudanese Henna) Leaves Extracts against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa among Recurrent Urinary Tract Infection patients in Omdurman Military Hospital

Author : Hanaa Abdalla Mohamed Elgailany and Yousif Fadlalla Hamed Elnil

Background: Lawsonia inermis (Sudanese Henna) a perennial plant that has the ability to grow in various environments in Sudan and traditionally uses as cosmetically for skin dye and hair and rich in antimicrobial agents that can be used for discovery of new classes of compounds that help in treatment of bacterial resistance strains. Methods: A total of 100 urine samples were collected from patient with recurrent urinary tract infection and inoculated on to CLED agar. Leaves of Lawsonia inermis were extracted by methanol and water and tested for its antibacterial activity using agar diffusion method whilst the phytochemical analysis of L.inermis leaves methanol extract using quantitative gas chromatoghraphy method. Results: Out of 32 isolated bacteria, 4 were Staphylococcus aureus (12.5%), 16 Escherichia coli (50%) and 3 Pseudomonas aeruginosa (9.4%). The reminder 9 (28%) were other bacteria. The studied methanol and water leavse extracts of L. inermis displayed various degree antibacterial activities were tested against isolated bacteria with minimum inhibitory concentration (MIC) between (6.25-25mg/ml), whilst water extract displayed no activity against E. coli and MDR E. coli. Gas chromatography analysis revealed that 51 chemical compounds of L. inermis (Henna). Conclusion: The results of the present study support that Lawsonia inermis has antibacterial activity against S. aureus, E. coli, P. aeruginosa, MRSA and MDR E. coli.

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