Clastogenic effect of selected south Indian medicinal plants by micronucleus assay A comparative evaluation

Author : Litty Joseph, K. K. Srinivasan, Jyoti Harindran

Micronucleus test, one of the most popular and promising tests on ecotoxicology, represents a cytogenetic indicator of DNA damage for over 30 years. The test procedure used in this study was described in the French Standard and evaluated the number of micro nucleated red blood cells (RBCs) in Zekarana keralenisis larvae treated with the of petroleum ether and ethanolic extracts of Justicia simplex, Myxopyrum smilacifolium, Memecylon malabaricum and Litzea lingustrina for 24 and 48h. The results indicated that petroleum ether extract of Justicia simplex showing the highest genotoxicity, especially at high concentration. Meanwhile, larvae exposed to cyclophosphamide exhibited significant genotoxicity. The MNT in bone-marrow and peripheral blood erythrocytes is one of the best established in vivo cytogenetic assays in the field of genetic toxicology, providing a convenient and reliable index of both chromosome breakage and chromosome loss.

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