Article

Development and validation of HPLC method for estimation of betulinic acid

Author : Meghraj Suryawanshi

A simple, specific, and economic HPLC method has been developed to estimate betulinic acid. The λmax of betulinic acid was found to be 210 nm. Linearity in the concentration range of 25-150 µg/ml was found to be exhibiting a good correlation coefficient (R² =0.999). The developed method was validated statistically to demonstrate linearity, accuracy, precision, LOD as well as LOQ. The validation parameters were selected as per the ICH [Q2 (R1)] guideline. The results of the study proved the applicability of the present method in routine analysis of betulinic acid.


Full Text Attachment

Creative Commons License World Journal of Pharmaceutical Science is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Based on a work at www.wjpsonline.org. Permissions beyond the scope of this license may be available at www.wjpsonline.org.