Development and validation of HPTLC method for simultaneous estimation of atorvastatin calcium and Vitamin D3 in pharmaceutical dosage form
HPTLC method was developed and validated for simultaneous estimation of Atorvastatin calcium and Vitamin D3 in their combined dosage form. Separation was achieved on aluminum plates precoated with silica gel 60F254 as stationary phase and mixture of Toluene: Ethyl acetate: Methanol: Glacial Acetic Acid (7:2:1:0.1 v/v/v/v) as a mobile phase. For simultaneous estimation of both drugs, photometric evaluation was performed at 262 nm. The drugs were satisfactorily resolved with Rf values of 0.38±0.02 and 0.83±0.02 for Atorvastatin calcium and Vitamin D3 respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (600-1400 ng/spot for both Atorvastatin calcium and Vitamin D3), precision (intra-day and inter-day)%RSD values were always less than 2.00 and accuracy (98.75±0.43% for Atorvastatin calcium and 98.79 ±0.33% for Vitamin D3).
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