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Effect of Azadirachta indica leaves on streptozotocin-induced diabetes mellitus and its associated complications

Author : Arvind Mishra, Sudeep Gautam, Savita Pal, Akansha Mishra, Arun Kumar Rawat, Rakesh Maurya, Arvind Kumar Srivastava

The present study focused on the effect of Azadirachta indica leaves on diabetes and its associated complications. Crude powder, aqueous, 95% and 50% ethanolic extracts of the leaves were administered to normal rats with sucrose load and streptozotocin-induced (STZ-induced) diabetic rats in a single dose study. 95% ethanolic extract was found most active and showed significant lowering of blood glucose in STZ-induced diabetic rats and also lowering of postprandial hyperglycemia in normal rats. Therefore, its various fractions were also tested in STZ-induced diabetic rats in single-dose experiment and on the basis of antihyperglycemic activity profile, the chloroform fraction was subjected to detailed study using in vitro and in vivo models. In multiple-dose studies, the chloroform fraction was found effective against various complications of diabetes, such as dyslipidemia and disturbed hepatic and renal functions in high fructose diet fed rats and STZ-induced diabetic rats. It also significantly increased the glucose uptake in treated L6 cells and increased the expression of IRS, AKT and GLUT 4 at both mRNA and protein level. The findings suggest that the chloroform fraction of 95% ethanolic extract of A.indica leaves is competent in combating various symptoms and complications of diabetes mellitus.


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