Effect of abiotic elicitation in some secondary compounds of callus Nerium oleander
Methanol extract of callus had high concentration of secondary metabolites concentration compared to leaves extract. Neritaloside compound concentration was significantly increasing at 0.5 mg /l concentration of KNO3, reaching to (383.45μg/ml) and the highest concentration significantly of Oleandrin was (362.85μg/ml) at 1.5 mg /l concentration of KNO3. Add CdCl2 at 2.5 mg/l lead to increase Neritaloside and Oleandrin significantly were reaching (131.61, 329.29μg/ml), respectively. Treatment by KNO3 and CdCl2 at all concentration led to decrease the fresh and dry weight of callus significantly.
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