Emergency of Helicobacter pylori resistance isolates obtained from Iraqi patients suffering Acute and chronic gastritis

Author : Ali Sajid Mohammed Ali, Israa M.S. Al-Kadmy, Munim Radwan Ali, SawsanSajid Al-Jubori

Helicobacter pylori considered one of the most common bacterial infections worldwide leading to acute or chronic gastritis, peptic ulceration and gastric cancer. In this study, infection was identified by Rapid Test Device (EOCtest) for qualitative detection of specific IgM and IgG antibodies in serum. According to EOCtest, 102\260 (39.2%) were positive .thirty five biopsies were obtained from the patient suffering acute and chronic gastritis .By using Colombia agar supplied with sheep blood, eight isolates were cultured then diagnosed using a Housekeeping for two cagA gene regions. Results of detecting antibiotic resistance genes including TEM, SHV, maxX, rrl ,acc3, acc6, aph(3)VI and rmtC showed that most of the isolate confer resistance to at least one of those genes .Four isolates harbored TEM gene confer resistance to penicillin's antibiotic, 5\8 isolate harbored SHV gene which resist broad spectrum B-lactams , 3 isolates harbored efflux pump maxX gene and one isolates showed positive result to rmtC gene responsible for 16S methyltransferaes of aminoglycoside. Some isolates demonstrate resistance to aminoglycoside by producing modifying enzymes acc3 &acc6 (7& 6 isolates, respectively). None of the isolate was clarithromycin resistant .This results might represent an indicator for emergence of resistance to β- Lactams, Aminogycoside group but not for clarithromycin.

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