Estimation and validation of entecavir in bulk and pharmaceutical dosage forms by UV spectrophotometry

Author : Jampani Subbarao, Reddy Rambabu, Suryadevara Vidyadhara

A simple, sensitive and economical UV-Spectrophotometric method was developed for the estimation of Entecavir in bulk and pharmaceutical dosage form. The absorption spectra in the proposed method, λmax of Entecavir was found to be 254nm. The calibration curve was linear over the concentration range of 3-18μg/ml with correlation coefficient of 0.999. A Percentage recovery of Entecavir ranges from 99.53-100.41%w/w indicating that the developed method is accurate and % RSD was less than 2 which indicates a good accuracy of the method. The limit of detection and limit of quantization were found to be 1.25μg/ml &3.81μg/ml. The proposed method will be suitable for the analysis of Entecavir in bulk and pharmaceutical formulation.

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