Evaluation of antioxidant activity of methanolic extract, fractions and pure compounds of Syzygium aromaticum
Antioxidant activity of methanolic extract of the Syzygium aromaticum, its fractions and pure compounds oleanolic acid (1), nigricin (2), 3, 4, 3′-tri-O-methylellagic acid (3), 2α-hydroxyoleanolic acid (4) and β-sitosterol-3-O-β-D-glucopyranoside (5) was determined by using 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical scavenging assay. The results showed that main aqueous (SA-MAQ), petroleum ether soluble (SA-PES), ethyl acetate soluble (SA-EAS), acetone soluble (SA-AS) and methanol soluble (SA-MS) fractions possess potent DPPH radical scavenging activity (EC50= 93.75 μg/ml). Compound (4) also showed potent activity with EC50 =93.75 μg/ml). This is the first report of systematic antioxidant activity of methanolic extract, its fractions and pure compounds isolated from S. aromaticum flower buds.
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