In vitro exposure to triclosan regulates hydroxysteroid dehydrogenase activity in epididymal sperm of goat
Triclosan, an antibacterial and antimicrobial agent, is widely used in pharmaceuticals, personal care and household products. The present study evaluated the effects of triclosan on sperm functional parameters and steroidogenic enzyme activity in goat epididymal sperm in vitro. Epididymal sperm of mature fertile goat were incubated with triclosan in Ringer’s phosphate solution. Experiments were divided into three groups, such as Group I: Triclosan at different concentrations (50, 100, 200 and 300 µM) for 5 h, Group II and III: Triclosan at 200 and 300 µM concentrations for different durations (1, 2, 3, 4 and 5 h). Epididymal sperm incubated with triclosan showed decline in sperm motility from 77 to 46% and sperm viability from 78% to 47% after 5 h of incubation in concentration-dependent manner. Activities of 3β-HSD decreased significantly at 200 and 300 µM, and 17β-HSD decreased significantly at 100, 200 and 300 µM of triclosan incubation for 5 h. Sperm when incubated with triclosan at 200 and 300 µM concentrations decreased the activities of 3β-HSD and 17β-HSD only after 3 h of incubation. In conclusion, triclosan affects the sperm functions as well as the male fertility by down regulating the activities of testicular steroidogenic enzymes.
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