Physicochemical and HPTLC analysis method for differentiation of Boerhavia diffusa from Boerhavia verticillata and Boerhavia repanda with application of PCA
Boerhavia is one of the highly polymorphic genus in Nyctaginaceae family. Boerhavia diffusa is a well-known Ayurvedic plant useful in many diseases like diabetes, inflammation, renal disorders, obesity, anemia etc. It is deliberately adulterated with other species like Boerhavia repanda and Boerhavia verticillata in pharmaceutical field.The study was planned to authenticate B. diffusa(BD) from other two species B. Verticillata(BV) and B. repanda(BR) with the help of its physicochemical and high performance thin layer chromatographic (HPTLC) profiling along with data mining technique as Principal Component Analysis (PCA).The whole plant powders of respective samples were subjected to physicochemical analysis and chromatographic study. The physicochemical results were analyzed by PCA. HPTLC analysis was performed in solvent system Toluene: Ethyl acetate: Methanol (7:1:2%v/v).The present findings suggest that physicochemical analysis can distinguish B. diffusa from B. verticillata and B. repanda by PCA on favor of thermal and solubility category variables. In HPTLC profiling 0.24 and 0.58 Rf spectra may be considered as marker spectra to identify the genuine BD. Physico-chemical, chromatographic fingerprinting along with PCA is developing the unorthodox reliable analytical method to quality monitoring of respective phylogenetic plant.
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