Physicochemical optimization of siRNA macromolecules with colloidal gold nanoparticles covered with cationic polymers
Gene silencing is facilitated by double-stranded small interfering RNA (siRNA) which is widely investigated as a potential therapeutic approach for many diseases especially hepatitis c virus disease. The uptake of siRNA is hindered by its rapid degradation both in vitro and in vivo. Therefore, we have investigated the stability of siRNA and its compatibility when condensed with cationic polymers and loaded onto gold nanoparticles. Physicochemical stability of the colloidaly stable particles was investigated using photon correlation spectroscopy, transmission electron microscopy, gel electrophoresis and ethidium bromide displacement assay. All methods produced nanosized particles were less than100 nm and depend on the molar ratio between the polymer repeating units and the siRNA nucleotides. The concentration of gold nanoparticles played a role in stabilizing the particles as well. The stability of the particles is enhanced by the type of the polymer used and by the molar ratios among siRNA and polymers @gold. The results revealed the efficiency of PEI to condense siRNA more efficiently than PLL.
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