Phytochemical investigation and standardization of aerial parts of Euphorbia Helioscopia L.
The present study has been designed to explore physicochemical properties and standardization of aerial parts of Euphorbia helioscopia L. by HPLC method. The various parts of plant have been reported in the treatment of number of pathological conditions such as anti-asthmatic, anti-oxidant, anti-tumor, etc. The active phytoconstituents such as flavonoids, alkaloids, phenolic compound, etc are majorly responsible for these activities. Physicochemical investigation was done by calculating extractive values (water and alcohol soluble), ash values (total ash, acid insoluble ash and water soluble ash), loss on drying and heavy metal content (arsenic, cadmium, lead and mercury). Further, extraction was done by percolation using petroleum ether and methanol successively. HPLC method for standardization of methanolic extract was developed to estimate % of markers present in extract by comparing with standard compounds. Results of present study confirmed percentage of these markers as chlorogenic acid 0.0001%, quercetin 0.00007%, quercetrin 0.081%, rutin 0.010% and luteolin glycoside 0.013%. Hence, it may be concluded that plant extract contains numbers of flavonoids, which further responsible for the various pharmacological activities.
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