Prevalence of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa from King Abdulaziz University Hospital in Jeddah

Author : Mahmoud Abdul-megead Yassien, Abdulrahman Mohammed Alahdal, Mahmoud Abdelkhalek Elfaky, Abdullah Adil Alzahrani, Suhaib Ali Bin Jabal, Rashad Dhaifallah Allahyani

Pseudomonas aeruginosa is an opportunistic pathogen associated with a wide range of nosocomial infections. A total of 75 P. aeruginosa clinical isolates were obtained from microbiology lab at King Abdulaziz University Hospital, Jeddah, Kingdom of Saudi Arabia. The antimicrobial susceptibility testing of these isolates was done using Kirby Bauer disc diffusion method as a preliminary screening method for detection of the β-lactam resistant isolates. The results showed that 80% (60/75) of the tested isolates were resistant to ceftizoxime. Ethylene diamine tetraacetic acid disc synergy test was applied on the resistant isolates to detect the metallo-β-lactamase producers. According to the obtained results, 45% (27 / 60 isolates) of the resistant isolates were found to be metallo-β-lactamase producers for which a confirmatory test for the presence of blaIMP-1 and/or blaVIM-1 genes was carried out by polymerase chain reaction amplification technique. The results revealed that 67.7% (18 isolates) and 33.3% (9 isolates) of metallo-β-lactamase producers had blaVIM-1 gene and blaIMP-1 gene, respectively.

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