Protective effect of Andrographis paniculata Nees and Vitamin-C in nicotine-induced oxidative stress in liver and kidney
Nicotine, a major toxic component of cigarette smoke has been identified as a major risk factor for different diseases. In the present study, we evaluated the protective effects of aqueous extract of Andrographis paniculata (AE-AP) and Vitamin C on lipid peroxidation and antioxidants status against nicotine treated liver and kidney toxicity. A group of male Wistar rats were induced by subcutaneous injection of vehicle, nicotine (2mg/kg body weight/day), nicotine plus AE-AP250 (250 mg/kg body weight/day), nicotine plus AE-AP500 (500 mg/kg body weight/day) and nicotine plus Vitamin C (5 mg/kg body weight/day) for the period of 15 days. Measurement of biochemical marker enzyme lactate dehydrogenase (LDH), lipid peroxidation (MDA), conjugated dienes and antioxidants were used to monitor the antiperoxidative effects of AE-AP and Vitamin C. The decreased biochemical marker enzyme as well as increased lipid peroxides and conjugated dienes in liver and kidney of nicotine treated rats was accompanied by a significant decrease in the levels of glutathione (GSH and GSSG) and the activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione-S-transferase (G-S-T), superoxide dismutase (SOD) and catalase (CAT). The results of the present study suggest that the administration of AE-AP and Vitamin C significantly supplement the biochemical marker enzyme, lipid peroxidation and enhanced the antioxidant status.
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