The relationship among mitochondrial dysfunction and antibiotic activities on Toxoplasma gondii in vitro
Toxoplasma gondii is the causative agent of toxoplasmosis, which current chemotherapies are self-limiting due to inability to eliminate the parasite and induce toxic effects. Then, new therapies are necessary and antibiotics in combinations with new drugs can be an alternative. Camptothecin, ciprofloxacin and tetracycline, known by inducing loss of mitochondrial activity, were used to verify their anti-T. gondii activities. Infected host cells were incubated with the antibiotics at different concentrations (1-100 uM) for 12 and 24 h. The parasites were eliminated from 5 uM, 12 h, with no toxic effect to host cell until 50 uM, 24 h. The reversibility assays confirmed that the camptothecin and tetracycline arrested the parasite replicative cycle, but not ciprofloxacin. The mitochondrial activity of parasite were gradually decreased during antibiotic treatments, although no morphological change was observed. In summary, the intracellular tachyzoites were eliminated from intracellular environment in presence of the antibiotics, possibly due to loss of its mitochondrial activity.
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